PE/Elab Fluor® 594 Anti-Mouse CD162 Antibody [4RA10]

Sizes: 50 Tests, 100 Tests, 100 Tests×2

Catalogue Numbers: E-AB-F1034P-50, E-AB-F1034P-100, E-AB-F1034P-100×2

Citations, Manuals and MSDS Available upon request.

Abbreviation: CD162

Target Synonym: P-selectin glycoprotein ligand 1, Selplg, PSGL-1, Selectin P ligand, CD162,

Conjugation: PE/Elab Fluor® 594

Host: Rat

Species Reactivity: Mouse

Application: FCM

Usage: Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Isotype: Rat IgG1, κ

Clone No.: 4RA10

UNIProt ID: Q62170

Background: The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

Concentration: 5 μL/Test

Control Catalogue Number: E-AB-F09822P

Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

GeneID: 20345

Storage: This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.

Shipping: Ice bag

Research Use Only