Human GSTa5 (Glutathione S Transferase Alpha 5) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1716-48, ELK1716-96, ELK1716-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: GST-a5; Glutathione S-Transferase A5; GST class-alpha member 5

Assay Type: Sandwich

Sensitivity: 0.238 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research Areas: Enzyme and Kinase; Tumor immunity; Hepatology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GSTa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GSTa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GSTa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GSTa5 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q7RTV2

Research Use Only