CD116/CSF2RA Recombinant Protein

Catalogue Number: NCP0252-500, NCP0252-1000

Sizes: 500ug, 1mg

Swiss-Prot: P15509

Host: E.coli

Tag: His-tag

AA Sequence: GAAAAGAGCGATCTGCGTACCGTGGCACCGGCAAGCAGCCTGAATGTTCGCTTCGATAGCCGCACCATGAATCTGAGCTGGGATTGCCAGGAAAATACCACCTTCAGTAAATGCTTCCTGACCGATAAAAAAAATCGTGTTGTGGAACCGCGCCTGAGTAATAATGAATGTAGCTGCACCTTCCGTGAAATCTGTCTGCATGAAGGCGTTACCTTCGAAGTGCATGTGAATACCAGCCAGCGTGGCTTCCAGCAGAAACTGCTGTATCCGAATAGTGGCCGTGAAGGTACCGCCGCACAGAACTTCAGTTGCTTCATCTATAATGCAGATCTGATGAATTGTACCTGGGCACGTGGTCCGACCGCACCGCGTGATGTGCAGTACTTCCTGTATATTCGCAATAGTAAACGCCGCCGCGAAATTCGCTGTCCGTATTATATTCAGGATAGTGGTACCCATGTGGGCTGTCATCTGGATAATCTGAGTGGTCTGACCAGTCGCAATTACTTCCTGGTTAATGGTACCAGTCGTGAAATTGGTATTCAGTTCTTCGATAGCCTGCTGGATACCAAAAAAATTGAACGCTTCAATCCGCCGAGTAATGTTACCGTGCGCTGCAATACCACCCATTGCCTGGTTCGTTGGAAACAGCCGCGCACCTATCAGAAACTGAGCTATCTGGACTTCCAGTATCAGCTGGATGTGCATCGTAAAAATACCCAGCCGGGTACCGAAAATCTGCTGATTAATGTTAGCGGTGATCTGGAAAATCGTTATAACTTCCCGAGTAGCGAACCGCGTGCAAAACATAGCGTGAAAATTCGTGCCGCAGATGTGCGTATTCTGAATTGGAGCAGTTGGAGTGAAGCAATTGAATTCGGTAGTGATGATGGC

Restriction Sites: NdeI-XhoI

Background: Granulocyte-macrophage colony-stimulating factor receptor subunit alpha (GM-CSF Receptor α), also known as CSF2RA and CD116, is a type 1 transmembrane protein with low affinity for GM-CSF. GM-CSF Receptor α is primarily expressed on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells. GM-CSF Receptor α forms a heterodimeric receptor complex with GM-CSF Receptor β, creating the high affinity GM-CSF receptor. Upon dimerization and ligand binding, the β subunit becomes phosphorylated and transduces signals via Jak/STAT and MAPK pathways for cell proliferation, differentiation, and survival. Oligomerization of the high affinity GM-CSF Receptor α/β complex is required for optimal intracellular signal transduction. Soluble GM-CSF Receptor α, either secreted or cleaved from the cell surface, can competitively bind GM-CSF, preventing membrane receptor signaling. Multiple isoforms of GM-CSF Receptor α have been described.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~33kDa

Notes: For research use only, not for use in diagnostic procedure.