General Information

1. Product Name: 1M Tris-HCl (pH 7.5)

2. Product Description: A 1M solution of Tris-HCl (Tris(hydroxymethyl)aminomethane hydrochloride), adjusted to a pH of 7.5, used as a buffer in various molecular biology and biochemical applications.

Physical and Chemical Characteristics

1. Appearance: Clear, colorless solution

2. pH: 7.5 ± 0.1

3. Concentration: 1M ± 10%

4. Sterility: Sterile filtered or autoclaved

Specifications

1. Assay: ≥99% (HPLC)

2. pH: 7.5 ± 0.1

3. Concentration: 1M ± 10%

4. Endotoxin: ≤0.1 EU/mL

5. Sterility: Sterile filtered or autoclaved

6. Particulate Matter: ≤10 particles/mL (≥ 10 μm)

Composition

1. Tris-HCl: 1M

2. Hydrochloric Acid (HCl): Used for pH adjustment

Applications

1. Molecular Biology: Used as a buffer in various molecular biology applications, such as PCR, DNA sequencing, and protein purification.

2. Biochemistry: Used as a buffer in various biochemical applications, such as enzyme assays and protein stability studies.

3. Cell Culture: Used as a buffer in cell culture media to maintain physiological pH.

Storage and Handling

1. Storage: Store at 2-8°C.

2. Handling: Handle with care, as the solution may be irritating to skin and eyes.

3. Shelf Life: 12 months from the date of manufacture.