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Wallerstein Agar is used for selective isolation and enumeration of bacteria encountered in breweries and industrial fermentations.
Cycloheximide is omitted from the original formulation
Formula
|
g/l
|
| Bromocresol green | 0.022 |
| Calcium chloride | 0.125 |
| Casein hydrolysate | 5 |
| Glucose | 50 |
| Ferric chloride | 0.0025 |
| Magnesium sulfate | 0.125 |
| Manganese sulfate | 0.0025 |
| Potassium Dihydrogen phosphate | 0.55 |
| Potassium chloride | 0.425 |
| Yeast extract | 4 |
| Agar | 15 |
Final pH 7.0 ± 0.2 at 25°C
Suspend 75.3 gram powdered medium in 1 litre distilled water
Store dry at room temperature
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