Suspend 6.9 gram powdered medium in 1 litre distilled water 

Store dry at room temperature

Yeast Nitrogen Base without amino acids (YNB w/o AA) is used for selecting yeasts based on amino acid and carbohydrate requirements. In Yeast Nitrogen Base without amino acids, Ammonium sulphate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells.

Compared to full Yeast Nitrogen Base medium, Yeast Nitrogen Base without amino acids lacks Histidine, Methionine and Trypthophan. In combination with Drop Out medium supplement mixtures, Yeast Nitrogen Base without Amino acids provides an excellent medium to cultivate and to select auxothophic strains of yeast that requires the addition of essential nutrients like amino acids and/or vitamins.

The addition of a carbon source like glucose, galactose or raffinose is required. Synthetic Dextrose Minimal medium (SD medium) and Synthetic Complete medium (SC medium) are based upon Yeast Nitrogen Base without amino acids and are complemented with glucose, galactose and raffinose as carbon sources.

Yeast, such as Saccharomyces cerevisiae, can efficiently manage nitrogen scarcity by quickly taking up and catabolising amino acids, which allows for the effective utilisation of nitrogen sources and subsequent biosynthesis of essential compounds (Kevvai et al., 2016 - see publications tab). Additionally, yeast nitrogen base without amino acids has been employed in research to induce specific growth conditions, such as filamentous growth, aiding in the investigation of gene expression patterns and cellular responses.